Lipophilic Grape Seed Proanthocyanidin Exert Anti-Proliferative and Pro-apoptotic Effects on PC3 Human Prostate Cancer Cells and Suppresses PC3 Xenograft Tumor Growth in Vivo.

Lipophilic Grape Seed Proanthocyanidin Exert Anti-Proliferative and Pro-apoptotic Effects on PC3 Human Prostate Cancer Cells and Suppresses PC3 Xenograft Tumor Growth in Vivo.

J Agric Food Chem. 2018 Dec 18;:

Authors: Chen M, Yu SJ

Abstract
The in vitro anti-prostate cancer activity of lipophilic grape seed proanthocyanidin (LGSP) against PC3 cell line was evaluated by MTT assay, flow cytometry and immunoblot analysis. And in vivo anti-prostate cancer effect was evaluated by a PC3-derived mouse xenograft model via oral gavage LGSP. Ki67 and cleaved caspase 3 immunostaining were performed in tumor tissues. LGSP exhibited a strong inhibitory effect on PC3 cell proliferation by inducing apoptosis. Treatment with LGSP resulted in a G1 phase cell cycle arrest in PC3 cells, which further confirmed by decreasing the expression of cyclin D1 and CDK 4 and increasing the expression of the tumor suppressor p21 and p27. Furthermore, activation of cleaved fragments of caspases 3, caspases 9 and PARP indicated that LGSP-induced apoptosis is caspase-dependent. Upstream of caspase cascade, LGSP increased the cytochrome c release in cytoplasm. And after treatment with LGSP, the Bcl-2/Bax ratio also decreased in PC3 cells. In tumor studies, LGSP inhibited the growth of PC3-derived mouse xenografts by inhibiting tumor cell proliferation and inducing apoptosis. Our findings suggest that LGSP is an effective anti-prostate cancer component and deserves further study.

PMID: 30562012 [PubMed - as supplied by publisher]

from A via a.sfakia on Inoreader https://ift.tt/2SbM4CP