Fluorescent Ag clusters conjugated with anterior gradient-2 antigen aptamer for specific detection of cancer cells

Publication date: 15 May 2019

Source: Talanta, Volume 197

Author(s): Jinze Lan, Xiaoxia Wu, Liang Luo, Jing Liu, Lingyan Yang, Fu Wang

Abstract

Anterior gradient protein 2 homolog (AGR) is a potential tumor biomarker and plays an important role in tissue development and regeneration. The intracellular detection of AGR is rarely reported. By conjugating the AGR aptamer with a cytosine base sequence as Ag cluster template, a highly fluorescent probe (MA@AgNCs) was synthesized for targeting intracellular AGR. The MA@AgNCs display a maximum fluorescence peak at 565 nm, and possess an excellent quantum yield (QY = 87.43%), small size, great biocompatibility, low toxicity, and good stability. Moreover, the as synthesized MA@AgNCs show high specificity on recognizing breast cancer cells.

Graphical abstract

Scheme 1 The sketch of synthesis process and fluorescence cell imaging.

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