A coumarin-based colorimetric fluorescent probe for rapid response and highly sensitive detection of hydrogen sulfide in living cells

Publication date: 15 May 2019

Source: Talanta, Volume 197

Author(s): Yang Yang, Yan Feng, Yong Jiang, Fangzhou Qiu, Yingzhe Wang, Xuerui Song, Xiaoliang Tang, Guolin Zhang, Weisheng Liu

Abstract

Hydrogen sulfide (H₂S) plays a vital role in numerous biological processes in living organisms. To better understand its functions, a fluorescent probe to fast and sensitively detect H₂S is imminently needed. Keep this in mind, we reasonably designed probe DHC for detecting H₂S based on α, β-unsaturated ethanoylcoumarin fluorophore. The limit of detection (LOD) is found to be as low as 5 × 10⁻⁸ M, which is superior to most reported fluorescent probes to detect H₂S. Furthermore, the wide pH range of 4–11 makes it capable of application in biological systems. Most importantly, MTT assays and cell imaging experiments indicate that probe DHC has hypotoxicity and outstanding membrane permeability, which makes DHC successful imaging of H₂S in Baby Hamster Syrian Kidney (BHK) cells.

Graphical abstract

A coumarin-based colorimetric fluorescent probe for rapid response and highly sensitive detection of hydrogen sulfide in living cells.

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