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Τρίτη 24 Οκτωβρίου 2017

Osteogenesis of Crouzon-Mutated Cells in a Murine Model.

Crouzon syndrome is an autosomal-dominant congenital disease due to a mutation in the fibroblast growth factor receptor 2 protein. The purpose of this study is to evaluate wound-healing potential of Crouzon osteoblasts and adipose-derived stem cells (ADSCs) in a murine model. Parietal skull defects were created in Crouzon and mature wild-type (WT) CD-1 mice. One group of WT and Crouzon mice were left untreated. Another group was transplanted with both WT and Crouzon adipose-derived stem cells. Additional groups compared the use of a fibrin glue scaffold and periosteum removal. Skulls were harvested from each group and evaluated histologically at 8-week and/or 16-week periods. Mean areas of defect were quantified and compared via ANOVA F-test. The average area of defect after 8 and 16 weeks in untreated Crouzon mice was 15.37 +/- 1.08 cm2 and 16.69 +/- 1.51 cm2, respectively. The average area of the defect in untreated WT mice after 8 and 16 weeks averaged 14.17 +/- 1.88 cm2 and 14.96 +/- 2.26 cm2, respectively. WT mice with autologous ADSCs yielded an average area of 15.35 +/- 1.34 cm2 after 16 weeks while Crouzon mice with WT ADSCs healed to an average size of 12.98 +/- 1.89 cm2. Crouzon ADSCs transplanted into WT mice yielded an average area of 15.47 +/- 1.29 cm2 while autologous Crouzon ADSCs yielded an area of 14.22 +/- 3.32 cm2. ANOVA F-test yielded P = .415. The fibroblast growth factor receptor 2 mutation in Crouzon syndrome does not promote reossification of critical-sized defects in mature WT and Crouzon mice. Furthermore, Crouzon ADSCs do not possess osteogenic advantage over WT ADSCs. (C) 2017 by Mutaz B. Habal, MD.

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Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,00306932607174,alsfakia@gmail.com,

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