Biomass accumulation of Panax vietnamensis in cell suspension cultures varies with addition of plant growth regulators and organic additives

Publication date: Available online 15 September 2017
Source:Asian Pacific Journal of Tropical Medicine
Author(s): Tuan Tran Trong, Dieu-Hien Truong, Hoang Chinh Nguyen, Dieu-Thai Tran, Huyen-Trang Nguyen Thi, Giap Do Dang, Ho Nguyen Huu
ObjectiveTo evaluate the impact of plant growth regulators including kinetin (KN), benzyl adenine (BA) and naphthalene acetic acid (NAA), yeast extract (YE) and casein hydrolysate (CH) on biomass accumulation of Vietnamese ginseng Panax vietnamensis (P. vietnamensis) in cell suspension culture.MethodsCell suspension cultures were established from friable calluses derived from leaves and petioles of 3-year-old in-vitro P. vietnamensis plants. The cell suspension cultures were grown in Murashige and Skoog basal media supplemented with various concentrations of KN, BA, NAA, and YE and CH.ResultsAll tested factors generated an increase in the cell biomass of P. vietnamensis in suspension culture, but the impact of each varies depended on the factor type, concentration, and incubation period. Addition of 2.0 mg/L KN resulted in the largest biomass increase after 24 d, (57.0 ± 0.9) and (3.1 ± 0.1) mg/mL fresh and dry weight, respectively, whereas addition of BA or NAA produced optimum levels of Panax cell biomass at 1.0 and 1.5 mg/L, respectively. Addition of the elicitor YE led to a 1.4–2.4 fold increase in biomass of P. vietnamensis, while addition of CH enhanced biomass accumulation 1.8–2.6 fold.ConclusionsThe addition of each factor caused significant changes in biomass accumulation of P. vietnamensis. The largest biomass accumulation was from cultures grown in MS media containing 2.0 mg/L KN for 24 d. The outcome of the present study provides new insights into the optimal suspension culture conditions for studies on the in vitro cell biomass production of P. vietnamensis.



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