Irradiation by blue light in the presence of a photoacid confers changes to colony morphology of the plant pathogen Colletotrichum gloeosporioides

Publication date: September 2017 Source:Journal of Photochemistry and Photobiology B: Biology, Volume 174 Author(s): Ron Simkovitch, Oren Gajst, Einat Zelinger, Oded Yarden, Dan Huppert We used the photoacid 8-hydroxy-1,3,6-pyrenetrisulfonate (HPTS) that converts blue photons to acidic protons in water, with an efficiency of close to 100%, and determined that this treatment conferred changes to colony morphology of the plant pathogen Colletotrichum gloeosporioides. The time elapsed until hyphal collapse is noticed depends on both the laser intensity in mW/cm2, and the concentration of HPTS in the Agar hydrogel. The time elapsed until hyphal collapse is noticed varies by only ±8% at HPTS concentrations of 500μM and at lower concentrations of HPTS the variance increases as the inverse of ...

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