Nanozyme‐Catalyzed Cascade Reactions for Mitochondria‐Mimicking Oxidative Phosphorylation

Multiple enzyme‐involved cascade reactions for bioenergy yielding are necessary in natural oxidative phosphorylation. However, for vitro application, one of the great challenges is hampered by sensitivity of catalytic activity to environmental adaption. Here, we explore nanozyme‐catalyzed cascade reactions in assembled hybrid architecture to achieve mitochondria‐mimicking oxidative phosphorylation. To be specific, gold nanoparticle‐trapped hollow silica microspheres are synthesized to accomplish two enzyme‐like catalytic reactions, which transform glucose into gluconic acid in the presence of oxygen. As a result, a transmembrane proton gradient is generated, which drives natural ATP synthase reconstituted on the surface to convert ADP and inorganic phosphate into ATP. Such assembled architecture possesses a high activity of oxidative phosphorylation, comparable to the natural mitochondria. This work provides a new prototype of natural‐artificial hybrids for exploring bioenergy supply systems, holding great promise in ATP‐powered bioapplications.

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