Phosphinate-based mitochondria-targeted fluorescent probe for imaging and detection of endogenous superoxide in live cells and in vivo

Publication date: 15 May 2019

Source: Talanta, Volume 197

Author(s): Saipeng Huang, Xiaojie Zhang, Yangping Liu, Jiacheng Gui, Ren Wang, Lu Han, Hongying Jia, Libo Du

Abstract

Monitoring superoxide anion radical (O₂^•-) in live cells and in vivo is of great significance since O₂^•- is the precursor of other reactive oxygen species and has been closely associated with various physiological and pathological processes. Herein, we developed a novel mitochondria-targeted fluorescent probe PF-MitoSOX Green in which the phosphinate moiety is utilized to recognize O₂^•- with high sensitivity and selectivity. Confocal imaging results illustrated that PF-MitoSOX Green can not only detect intracellular O₂^•-, but also can conveniently visualize its production in cells and Caenorhabditis elegans. The present study illustrates that PF-MitoSOX Green provides a novel approach for imaging and detecting O₂^•- in live cells and in vivo.

Graphical abstract

A novel phosphinate-based fluorescent probe PF-MitoSOX was developed in which the phosphinate moiety is used to specifically sense O₂^•-. The fluorescence results illustrated that PF-MitoSOX can not only detect intracellular O₂^•-, but also can conveniently visualize its production in cells and C. elegans.

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