Evaluation of characteristic of human turbinate derived mesenchymal stem cells cultured in the serum free media.

Evaluation of characteristic of human turbinate derived mesenchymal stem cells cultured in the serum free media.

PLoS One. 2017;12(10):e0186249

Authors: Hwang SH, Lee W, Park SH, Lee HJ, Park SH, Lee DC, Lim MH, Back SA, Yun BG, Jeun JH, Lim JY, Kang JM, Kim SW

Abstract
We evaluated the effect of serum-free and xeno-cultivation (SFXFM) on the characterization, proliferation, and differentiation properties of human nasal stem cells (airway tissue; hTMSCs). hTMSCs were isolated from 10 patients, after which patient samples were separated into two groups, an SFXFM group and a control group. The control group was treated with bovine serum-containing medium. FACS analysis revealed that SFXFM-cultured hTMSCs maintained a characteristic mesenchymal stem cell phenotype. hTMSC proliferation was not influenced by SFXFM. In addition, upregulation of IL-8 and GM-CSF and downregulation of RANTES expression were shown in response to SFXFM. Moreover, two-lineage differentiation properties (osteocyte and adipocyte) of hTMSCs were enhanced under SFXFM. Finally, the genetic stability of SFXFM-cultured hTMSCs was demonstrated by normal karyotype results. SFXFM enables good expansion, multipotentiality, and normal genotype maintenance of MSCs. Moreover, this approach serves as a substitute to conventional media for the cultivation of capable MSCs for upcoming medical applications.

PMID: 29049314 [PubMed - in process]

from # All Medicine by Alexandros G. Sfakianakis via Alexandros G.Sfakianakis on Inoreader http://ift.tt/2yB2YDx
via IFTTT