Epidemiology of malaria and MSP-2 gene-based genetic diversity of Plasmodium falciparum from patients attending community health centre, Jiribam, Manipur

Indu Sharma, Appu Saikia, Paromita Chakraborty

Indian Journal of Medical Microbiology 2017 35(3):410-414

Two millilitres of peripheral blood was collected from 323 patients of different age groups and rapid diagnosis (RDT test) was performed. Parasite genomic DNA was extracted from whole blood and MSP-2 gene-based diversity and polymorphism was determined followed by restriction fragment length polymorphism analysis using Taq 1 and Vsp 1 digestion of each polymerase chain reaction product to analyse MSP-2 genotypes. Twenty-six sequences of P. falciparum MSP-2 gene were retrieved from the current GenBank database to represent strains from various geographical locations and were analysed for the Taq 1 and Vsp 1 enzyme restriction sites using in silico restriction digestion in Sequence Manipulation Suite, Version 2.

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